Anti-SPAK (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9UEW8
Gene Id	27347
Gene Symbols	STK39
Protein Name / Synonyms	SPAK (Center)
Clonality	Polyclonal
Preparation	This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.

Specificity

Predicted Reactivity

Rat

Other Names

STK39; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase; DCHT; Serine/threonine-protein kinase 39

NCBI Accession #

NP_037365.2

Other Accession #

O88506; Q9Z1W9

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59474

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysate from 293 cell line, using SPAK Antibody (A363)(Cat# 102-17734). 102-17734 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Western blot analysis of anti-SPAK Pab (Cat# 102-17734) in mouse liver tissue lysate. SPAK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	SPAK Antibody (A363) (Cat# 102-17734) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the SPAK antibody detected the SPAK protein (arrow).
	Western blot analysis of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363) (Cat# 102-17734). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.