Anti-SPHK1 (C-term R414) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9NYA1
Gene Id	8877
Gene Symbols	SPHK1
Protein Name / Synonyms	SPHK1 (C-term R414)
Clonality	Polyclonal
Preparation	This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-343 amino acids from the C-terminal region of human SPHK1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.

Specificity

Other Names

SPHK1; SPHK; SPK; Sphingosine kinase 1

NCBI Accession #

NP_001136073.1;NP_001136074.1;NP_068807.2;NP_892010.2

Other Accession #

Q8N632

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42518

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of SPHK1 Antibody (C-term R414) (Cat# 102-17200) in 293 cell line lysates (35ug/lane).SPHK1 (arrow) was detected using the purified Pab.
	SPHK1 Antibody (C-term R414)(Cat# 102-17200) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPHK1 Antibody (C-term R414) for immunohistochemistry. Clinical relevance has not been evaluated.
	Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (C-term R414) (Cat# 102-17200). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
	SPHK1 Antibody (C-term R414) (Cat# 102-17200) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products