Anti-SPI1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P17947
Gene Id	6688
Gene Symbols	SPI1
Protein Name / Synonyms	SPI1 (C-term)
Clonality	Polyclonal
Preparation	This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

SPI1; Transcription factor PU.1; 31 kDa-transforming protein

NCBI Accession #

NP_001074016.1;NP_003111.2

Other Accession #

Q6BDS1; Q6PKU1; P17433; NP_003111.2; NP_001074016.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31083

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody (C-term)(Cat# 102-12686/SA110831AS).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). SPI1 immunoreactivity is localized to Nucleus significantly.
	SPI1 Antibody (C-term) (Cat# 102-12686) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the SPI1 antibody detected the SPI1 protein (arrow).
	SPI1 Antibody (C-term) (102-12686)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SPI1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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