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Anti-SPINT1 Antibody
Catalog #: 144-06411
SPINT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43278 |
| Gene Id | 6692 |
| Gene Symbols | SPINT1 |
| Protein Name / Synonyms | SPINT1, HAI, HAI1, MANSC2, kunitz-type protease inhibitor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
56kDa/58kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SPINT1 antibody (144-06411) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (144-06411) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (144-06411) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SPINT1 antibody (144-06411). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.