3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SPP1 (C-term) Antibody
Catalog #: 102-16822
Rabbit Anti-Human SPP1 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P10451 |
| Gene Id | 6696 |
| Gene Symbols | SPP1 |
| Protein Name / Synonyms | SPP1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Specificity
Other Names
SPP1; BNSP; OPN; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin
NCBI Accession #
NP_000573.1;NP_001035147.1;NP_001035149.1;NP_001238758.1;NP_001238759.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35423
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of SPP1 Antibody (C-term) (Cat# 102-16822) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.