Anti-SPR Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	6697
Protein Name / Synonyms	SPR
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

SDR38C1 antibody, SPR antibody, sepiapterin reductase antibody, "short chain dehydrogenase/reductase family 38C, member 1 antibody", "sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

28 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 65 and 261 of SPR (Uniprot ID# P35270)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using SPR(107-11078) antibody at 1:200 dilution.

SPR antibody [N2C3] detects SPR protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SPR antibody [N2C3] (107-11078) dilution: 1:500.

SPR antibody [N2C3] immunoprecipitates SPR protein in IP experiments.
IP samples: A431 whole cell extract
A. Control with 4 ?g of preimmune Rabbit IgG
B. Immunoprecipitation of SPR protein by 4 ?g SPR antibody [N2C3] (107-11078)
5 % SDS-PAGE
The immunoprecipitated SPR protein was detected by SPR antibody [N2C3] (107-11078) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-11078 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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