Anti-SPRY4-Y75 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9C004
Gene Id	81848
Gene Symbols	SPRY4
Protein Name / Synonyms	SPRY4-Y75
Clonality	Polyclonal
Preparation	This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human SPRY4.
Concentration (lot specific)	0.430 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.

Specificity

Other Names

SPRY4; Protein sprouty homolog 4

NCBI Accession #

NP_001120968.1;NP_112226.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32541

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of Phospho-SPRY4-Y75.ctrl (Cat# 102-17350) in K562 cell line lysates (35ug/lane). SPRY4 (arrow) was detected using the purified Pab.
	SPRY4-Y75 Antibody (Cat# 102-17350) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	SPRY4-Y75 Antibody (Cat# 102-17350) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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