Anti-SQSTM1 (p62) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13501
Gene Id	8878
Gene Symbols	SQSTM1
Protein Name / Synonyms	SQSTM1 (p62) (C-term)
Clonality	Polyclonal
Preparation	This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Apoptosis, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

SQSTM1; ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62

NCBI Accession #

NP_001135770.1;NP_001135771.1;NP_003891.1

Other Accession #

O08623; Q64337

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

47687

Recommended Dilutions

IF: 1:50-100; WB: 1:1000; IHC: 1:50-100

	Fluorescent image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15010 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).
	Western blot analysis of SQSTM1 Antibody (C331) Pab (Cat# 102-15010) pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate. SQSTM1 Antibody (C331) (arrow) was detected using the purified Pab.
	Western blot analysis of SQSTM1 (arrow) using rabbit polyclonal Autophagy SQSTM1 (p62) Antibody (C-term ) (Cat# 102-15010). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (Cat# 102-15010) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.