3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SRC Antibody
Catalog #: 144-61493
Phospho-SRC-Y529 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P12931 |
| Gene Id | 6714 |
| Gene Symbols | SRC |
| Protein Name / Synonyms | SRC, ASV, SRC1, THC6, c-SRC, p60-Src, proto-oncogene tyrosine-protein kinase Src |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200
IHC 1:50 - 1:100
IF 1:100 - 1:200
Format
Calculated MW
59kDa/60kDa
Observed MW
60kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Y529 of human SRC
Modification
Phospho Y529
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-SRC-Y529 antibody (144-61493) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-SRC-Y529 antibody (144-61493).
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-SRC-Y529 antibody (144-61493).
Expiration:
12 months from the date of shipment when stored properly.