Rabbit Anti-SRC (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P12931 |
| Gene Id | 6714 |
| Gene Symbols | SRC |
| Protein Name / Synonyms | SRC (N-term) |
| Clonality | Polyclonal |
| Preparation | This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SRC. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.
Specificity
Other Names
SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src
NCBI Accession #
NP_005408.1;NP_938033.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
59835
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | Western blot analysis of lysates from MCF-7,rat PC-12,mouse C2C12 cell line (from left to right),using SRC Antibody (R14)(Cat# 102-17583).102-17583 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Confocal immunofluorescent analysis of SRC Antibody (N-term)(Cat# 102-17583) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.