3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SREBF1 (Center) Antibody
Catalog #: 102-18005
Rabbit Anti-Human SREBF1 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P36956 |
| Gene Id | 6720 |
| Gene Symbols | SREBF1 |
| Protein Name / Synonyms | SREBF1 (Center) |
| Clonality | Polyclonal |
| Preparation | This SREBF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-378 amino acids from the Central region of human SREBF1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.
Specificity
Predicted Reactivity
Mouse, Rat, Hamster
Other Names
SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; Class D basic helix-loop-helix protein 1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1
NCBI Accession #
NP_001005291.1;NP_004167.3
Other Accession #
P56720; Q9WTN3; Q60416
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
121675
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of SREBF1 Antibody (Center) (Cat# 102-18005) in MDA-MB435 cell line lysates (35ug/lane). SREBF1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with SREBF1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | SREBF1 Antibody (Center) (Cat# 102-18005) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.