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Anti-SREBF2 (Center) Antibody
Catalog #: 102-10748
Rabbit Anti-Human SREBF2 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q12772 |
| Gene Id | 6721 |
| Gene Symbols | SREBF2 |
| Protein Name / Synonyms | SREBF2 (Center) |
| Clonality | Polyclonal |
| Preparation | This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the Central region of human SREBF2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.
Specificity
Other Names
SREBF2; BHLHD2; SREBP2; Sterol regulatory element-binding protein 2; Class D basic helix-loop-helix protein 2; Sterol regulatory element-binding transcription factor 2; Processed sterol regulatory element-binding protein 2
NCBI Accession #
NP_004590.2
Other Accession #
NP_004590.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
123688
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50
 | Fluorescent image of U251 cell stained with SREBF2 Antibody (Center)(Cat# 102-10748).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SREBF2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).SREBF2 immunoreactivity is localized to Nucleus and Nucleolus significantly. |
 | SREBF2 Antibody (Center) (Cat# 102-10748) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow). |
 | SREBF2 antibody (Center) (Cat# 102-10748) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SREBF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.