Anti-SRF Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P11831
Gene Id	6722
Gene Symbols	SRF
Protein Name / Synonyms	SRF, MCM1, serum response factor
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

51kDa

Observed MW

70kDa

Immunogen

A synthetic phosphorylated peptide around S103 of human SRF (NP_003122.1).

Modification

Phospho S103

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using Phospho-SRF-S103 antibody (144-61542) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - Phospho-SRF-S103 pAb (144-61542)

Expiration:

12 months from the date of shipment when stored properly.

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