Anti-STAM (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q92783
Gene Id	8027
Gene Symbols	STAM
Protein Name / Synonyms	STAM (C-term)
Clonality	Polyclonal
Preparation	This STAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human STAM.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). STAM for 'signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540-amino acid protein precipitated by anti-phosphotyrosine. Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.

Specificity

Predicted Reactivity

Mouse

Other Names

STAM; STAM1; Signal transducing adapter molecule 1

NCBI Accession #

NP_003464.1

Other Accession #

P70297

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59180

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-STAM Antibody (C-term) (Cat# 102-15009) in Hela cell line lysates (35ug/lane). STAM (arrow) was detected using the purified Pab.
	Western blot analysis of STAM (arrow) using rabbit polyclonal STAM Antibody (P341) (Cat# 102-15009). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAM gene.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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