Anti-STAP2 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	55620
Protein Name / Synonyms	STAP2, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

BKS antibody, FLJ20234 antibody, STAP2 antibody, breast tumor kinase substrate antibody, signal-transducing adaptor protein-2 antibody, brk kinase substrate antibody, BRK substrate antibody, signal-transducing adaptor protein 2 antibody, signal transducing adaptor family member 2 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

45 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 340 and 403 of STAP2 (Uniprot ID# Q9UGK3)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HCT116, using STAP2(107-10601) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using STAP2(107-10601) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: HCT116 10% SDS PAGE
107-10601 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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