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Anti-STAR Antibody
Catalog #: 107-10553
STAR antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 6770 |
| Protein Name / Synonyms | STAR |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
STARD1 antibody, STAR antibody, START domain containing 1 antibody, StAR-related lipid transfer (START) domain containing 1 antibody, cholesterol trafficker antibody, mitochondrial steroid acute regulatory protein antibody, steroid acute regulatory protein antibody, steroidogenic acute regulatory protein, mitochondrial antibody, steroidogenic acute regulator antibody, START domain-containing protein 1 antibody, steroidogenic acute regulatory protein antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 15 and 268 of STAR (Uniprot ID# P49675)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
STAR antibody detects STAR protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAR protein stained by STAR antibody (107-10553) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STAR protein stained by STAR antibody (107-10553) diluted at 1:500.
Blue: Hoechst 33342 staining.
STAR antibody detects STAR protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
STAR antibody (107-10553) dilution: 1:500.
Sample: Paraffin-embedded hepatoma.
STAR antibody (107-10553) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
107-10553 diluted at 1:500
A: Mouse liver
12% SDS PAGE
107-10553 diluted at 1:500
Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
107-10553 diluted at 1:1000
A: Molt-4
12% SDS PAGE
107-10553 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.