Anti-STEP / PTPN5 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P54829
Gene Id	84867
Gene Symbols	PTPN5
Protein Name / Synonyms	STEP / PTPN5 (N-Term)
Clonality	Polyclonal
Preparation	This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.

Specificity

Other Names

PTPN5; Tyrosine-protein phosphatase non-receptor type 5; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase

NCBI Accession #

NP_001035059.1;NP_001265165.1;NP_001265167.1;NP_001265168.1;NP_008837.1;NP_116170.3

Other Accession #

P54830

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

63538

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of hSTEP-Q155 (Cat# 102-17868) in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab
	Western blot analysis of hSTEP-Q155 (Cat# 102-17868) in HepG2 cell line lysates (35ug/lane). STEP (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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