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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 10963 |
| Protein Name / Synonyms | STIP1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HOP antibody, IEF-SSP-3521 antibody, P60 antibody, STI1 antibody, STI1L antibody, STIP1 antibody, hsc70/Hsp90-organizing protein antibody, renal carcinoma antigen NY-REN-11 antibody, Hsp70/Hsp90-organizing protein antibody, NY-REN-11 antigen antibody, transformation-sensitive protein IEF SSP 3521 antibody, stress-induced-phosphoprotein 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
63 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 300 of STIP1 (Uniprot ID# P31948)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using STIP1(107-10364) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using STIP1(107-10364) antibody at 1:100 dilution.
STIP1 antibody detects STIP1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
STIP1 antibody (107-10364) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
STIP1 antibody (107-10364) dilution: 1:1000
Sample(30 µg of whole cell lysate)
A:Hep G2
B:MOLT4
7.5% SDS PAGE
107-10364 diluted at 1:5000
A:Hep G2
B:MOLT4
7.5% SDS PAGE
107-10364 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
