Anti-STK11 (LKB1) (N-term I29) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15831
Gene Id	6794
Gene Symbols	STK11
Protein Name / Synonyms	STK11 (LKB1) (N-term I29)
Clonality	Polyclonal
Preparation	This STK11 (LKB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-44 amino acids from the N-terminal region of human STK11 (LKB1).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

STK11, is a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in STK11 have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms.

Specificity

Predicted Reactivity

Mouse, Rat, Chicken, Xenopus

Other Names

STK11; LKB1; PJS; Serine/threonine-protein kinase STK11; Liver kinase B1; Renal carcinoma antigen NY-REN-19

NCBI Accession #

NP_000446.1

Other Accession #

Q91604; D4AE59; Q9WTK7; Q0GGW5

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48636

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Western blot analysis of STK11 (arrow) using rabbit polyclonal STK11 Antibody (Cat# 102-17203). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2) (Origene Technologies).
	STK11 Antibody (I29) (Cat# 102-17203) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the STK11 antibody detected the STK11 protein (arrow).
	STK11 (LKB1) Antibody (N-term I29) (Cat# 102-17203) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of STK11 (LKB1) Antibody (N-term I29)(Cat# 102-17203) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).