STK25 antibody [N1C1], 0.38 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10494 |
| Protein Name / Synonyms | STK25 |
| Concentration (lot specific) | 0.38 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp686J1430 antibody, SOK1 antibody, YSK1 antibody, STK25 antibody, serine/threonine-protein kinase 25 antibody, ste20/oxidant stress response kinase 1 antibody, "serine/threonine kinase 25 (STE20 homolog, yeast) antibody", "Ste20, yeast homolog antibody", SOK-1 antibody, ste20-like kinase antibody, sterile 20 (oxidant stress response kinase 1, yeast Sps1/Ste20-related kinase 1) antibody, sterile 20/oxidant stress-response kinase 1 antibody, serine/threonine kinase 25 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
48 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 231 of STK25 (Uniprot ID# O00506)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using STK25(107-10694) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using STK25(107-10694) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
107-10694 diluted at 1:1000
A: Hela S3
10% SDS PAGE
107-10694 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.