Anti-STK4 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	6789
Protein Name / Synonyms	STK4
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp686A2068 antibody, KRS2 antibody, MST1 antibody, YSK3 antibody, STK4 antibody, mammalian sterile 20-like 1 antibody, kinase responsive to stress 2 antibody, serine/threonine-protein kinase Krs-2 antibody, MST-1 antibody, "dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2)) antibody", STE20-like kinase MST1 antibody, mammalian STE20-like protein kinase 1 antibody, serine/threonine-protein kinase 4 antibody, serine/threonine kinase 4 antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

56 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 324 and 418 of STK4 (Uniprot ID# Q13043)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4(107-10831) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

STK4 antibody immunoprecipitates STK4 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 µg HepG2 whole cell extract
B. Control with 4 µg of preimmune Rabbit IgG
C. Immunoprecipitation of STK4 protein by 4 µg STK4 antibody (107-10831)
7.5 % SDS-PAGE
The immunoprecipitated STK4 protein was detected by STK4 antibody (107-10831) diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10831 diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MST1(107-10831) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
STK4 antibody
107-10831 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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