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Anti-SUCLG1 Antibody
Catalog #: 107-10822
SUCLG1 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8802 |
| Protein Name / Synonyms | SUCLG1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ21114 antibody, FLJ43513 antibody, GALPHA antibody, MTDPS9 antibody, SUCLA1 antibody, SUCLG1 antibody, succinyl-CoA synthetase subunit alpha antibody, succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial antibody, succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial antibody, SCS-alpha antibody, succinate-CoA ligase, alpha subunit antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 346 of SUCLG1 (Uniprot ID# P53597)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: SUCLG1 protein stained by SUCLG1 antibody (107-10822) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: SUCLG1 protein stained by SUCLG1 antibody (107-10822) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SUCLG1(107-10822) antibody at 1:100 dilution.
Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
107-10822 diluted at 1:10000
A: mouse brain
10% SDS PAGE
107-10822 diluted at 1:10000
SUCLG1 antibody detects SUCLG1 protein by Western blot analysis.
A.50 µg rat brain lysate/extract
10 % SDS-PAGE
SUCLG1 antibody (107-10822) dilution: 1:3000
A.50 µg rat brain lysate/extract
10 % SDS-PAGE
SUCLG1 antibody (107-10822) dilution: 1:3000
Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10822 diluted at 1:1000
A: A431
10% SDS PAGE
107-10822 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.