Anti-SUMO1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P63165
Gene Id	7341
Gene Symbols	SUMO1
Protein Name / Synonyms	SUMO1 (N-term)
Clonality	Polyclonal
Preparation	This SUMO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SUMO1.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

SUMO1; SMT3C; SMT3H3; UBL1; Small ubiquitin-related modifier 1; GAP-modifying protein 1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1

NCBI Accession #

NP_001005781.1;NP_001005782.1;NP_003343.1

Other Accession #

Q5I0H3; A7WLH8; P63166; Q5E9D1; NP_001005781.1; NP_003343.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11557

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Fluorescent image of A549 cell stained with SUMO1 Antibody (N-term)(Cat# 102-12113).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).SUMO1 immunoreactivity is localized to Nuclear Membrane significantly.
	SUMO1 Antibody (N-term) (Cat# 102-12113) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SUMO1 antibody detected the SUMO1 protein (arrow).
	SUMO1 Antibody (N-term) (Cat# 102-12113)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SUMO1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products