Anti-SUMO2/3 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P55854
Gene Id	6612
Gene Symbols	SUMO3
Protein Name / Synonyms	SUMO2/3 (C-term)
Clonality	Polyclonal
Preparation	This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Specificity

Predicted Reactivity

Rat, Zebrafish, Bovine, Chicken, Hamster, Pig, Monkey, Xenopus

Other Names

SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B

NCBI Accession #

NP_008867.2

Other Accession #

Q7SZ22; Q5XIF4; Q9Z172; Q6DI05; Q17QV3; P61959; P61958; P61957; Q2PFW2; P61956; Q6DHL4; Q6LDZ8; Q5ZJM9; P61955; Q6NV25; Q6GPW2; Q7ZTK7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11637

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from 293T, Hela, HL-60, Jurkat cell lines and rat liver tissue lysate (from left to right), using SUMO2/3 Antibody (Q65) (Cat# 102-11468). 102-11468 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	SUMO2/3 Antibody (C-term) (Cat# 102-11468) western blot analysis in U251 cell lysate,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates that the SUMO2/3 antibody detected SUMO2/3 protein (arrow).
	SUMO2/3 Antibody (C-term) (Cat# 102-11468) western blot analysis in Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).
	The anti-SUMO2/3 C-term Pab (Cat# 102-11468) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.
	Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65) (Cat# 102-11468). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.