Anti-SUMO2/3 (C-term E69) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P55854
Gene Id	6612
Gene Symbols	SUMO3
Protein Name / Synonyms	SUMO2/3 (C-term E69)
Clonality	Polyclonal
Preparation	This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-84 amino acids from the C-terminal region of human SUMO2/3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Hamster, Pig, Monkey, Xenopus

Other Names

SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B

NCBI Accession #

NP_008867.2

Other Accession #

Q7SZ22; Q5XIF4; Q9Z172; Q6DI05; Q17QV3; P61959; P61958; P61957; Q2PFW2; P61956; Q6DHL4; Q6LDZ8; Q5ZJM9; P61955; Q6NV25; Q6GPW2; Q7ZTK7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11637

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	SUMO2/3 Antibody (C-term E69) (Cat# 102-11464) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).
	Fluorescent confocal image of Hela cell stained with SUMO2/3 Antibody (C-term E69)(Cat# 102-11464).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO2/3 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).SUMO2/3 immunoreactivity is localized to Nucleus significantly.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69) (Cat# 102-11464), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products