Anti-SUMO2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P61956
Gene Id	6613
Gene Symbols	SUMO2
Protein Name / Synonyms	SUMO2 (C-term)
Clonality	Polyclonal
Preparation	This SUMO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-93 amino acids from the C-terminal region of human SUMO2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SUMO2 is a member of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Hamster, Pig, Monkey, Xenopus

Other Names

SUMO2; SMT3A; SMT3H2; Small ubiquitin-related modifier 2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A

NCBI Accession #

NP_001005849.1;NP_008868.3

Other Accession #

Q7SZ22; Q5XIF4; Q9Z172; P55854; Q6DI05; Q5ZHQ1; Q17QV3; P61959; P61958; P61957; Q2PFW2; Q6DHL4; Q6LDZ8; Q5ZJM9; P61955; Q6NV25; Q6GPW2; Q7ZTK7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

10871

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from 293T, HL-60, Jurkat cell line (from left to right), using SUMO2 Antibody(Cat# 102-11753). 102-11753 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	The SUMO2 C-term Antibody (Cat# 102-11753) is used in Western blot to detect SUMO2 in GST-SUMO2 fusion protein (lane 1) and HL60 cell lysate (lane 2).
	Western blot analysis of SUMO2 (arrow) using rabbit polyclonal SUMO2 Antibody (Cat# 102-11753). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2 gene.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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