Anti-SUMO4 (V55 Mutant) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6EEV6
Gene Id	387082
Gene Symbols	SUMO4
Protein Name / Synonyms	SUMO4 (V55 Mutant)
Clonality	Polyclonal
Preparation	This SUMO4 antibody is generated from rabbits immunized with a KLH conjugated peptide CEPRGLS(V)KQIRFRFG selected from human SUMO4. This antibody is affinity purified using peptides CEPRGLS(V)KQIRFRFG (positive selection) and CEPRGLS(M)KQIRFRFG (negative selection).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.

Specificity

Other Names

SUMO4; SMT3H4; Small ubiquitin-related modifier 4; Small ubiquitin-like protein 4

NCBI Accession #

NP_001002255.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

10685

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of SUMO4 Antibody (V55 Mutant) (Cat# 102-11676) in HepG2 cell line lysate (35ug/lane). SUMO4 mutant (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO4 antibody (V55 Mutant) (Cat# 102-11676), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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