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Anti-SYK Antibody
Catalog #: 144-02123
SYK Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P43405 |
| Gene Id | 6850 |
| Gene Symbols | SYK |
| Protein Name / Synonyms | SYK, p72-Syk, tyrosine-protein kinase SYK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
69kDa/72kDa
Observed MW
69kDa
Immunogen
Recombinant protein of human SYK
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SYK antibody (144-02123) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using SYK Antibody (144-02123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SYK Antibody (144-02123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SYK Antibody (144-02123) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SYK Antibody (144-02123) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SYK antibody (144-02123) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.