Anti-SYVN1 (HRD1) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q86TM6
Gene Id	84447
Gene Symbols	SYVN1
Protein Name / Synonyms	SYVN1 (HRD1) (C-term)
Clonality	Polyclonal
Preparation	This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.

Specificity

Other Names

SYVN1; HRD1; KIAA1810; E3 ubiquitin-protein ligase synoviolin; Synovial

NCBI Accession #

NP_115807.1;NP_757385.1

Other Accession #

Q9DBY1; Q8N6E8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

67685

Recommended Dilutions

IF: 1:200; WB: 1:1000; IHC: 1:50-100

	Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15011 SYVN1 (HRD1) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min).
	Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (RayBiotech # 102-15011, 1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).
	Western blot analysis of SYVN1 (HRD1) Antibody (C-term) (Cat# 102-15011) in T47D cell line lysates (35ug/lane). HRD1 (arrow) was detected using the purified Pab.
	The anti-HRD1 Pab (Cat# 102-15011) is used in Western blot to detect HRD1 in mouse kidney tissue lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term)(Cat# 102-15011), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.