Rabbit Anti-Human TAGLN (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01995 |
| Gene Id | 6876 |
| Gene Symbols | TAGLN |
| Protein Name / Synonyms | TAGLN (N-term) |
| Clonality | Polyclonal |
| Preparation | This TAGLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human TAGLN. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
TAGLN; SM22; WS3-10; Transgelin; 22 kDa actin-binding protein; Protein WS3-10; Smooth muscle protein 22-alpha
NCBI Accession #
NP_001001522.1;NP_003177.2
Other Accession #
P31232; P37804; Q9TS87
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
22611
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of TAGLN antibody (N-term) (Cat# 102-16881) in SK-Br-3 cell line lysates (35ug/lane). TAGLN (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAGLN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MDA-MB468 cells using TAGLN Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.