Anti-TBRG4 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	9238
Protein Name / Synonyms	TBRG4
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CPR2 antibody, FASTKD4 antibody, KIAA0948 antibody, TBRG4 antibody, FAST kinase domains 4 antibody, protein TBRG4 antibody, cell cycle progression 2 protein antibody, FAST kinase domain-containing protein 4 antibody, H_TD2522F11.8 antibody, cell cycle progression restoration protein 2 antibody, cell cycle progression protein 2 antibody, transforming growth factor beta regulator 4 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

71 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 270 (Q969Z0) of TBRG4

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

TBRG4 antibody detects TBRG4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TBRG4 protein stained by TBRG4 antibody (107-10681) diluted at 1:500.
Blue: Hoechst 33343 staining.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TBRG4(107-10681) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:A431
B:Hep G2
7.5% SDS PAGE
107-10681 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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