Anti-TBX21 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UL17
Gene Id	30009
Gene Symbols	TBX21
Protein Name / Synonyms	TBX21 (N-term)
Clonality	Polyclonal
Preparation	This TBX21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human TBX21.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	IF, Western Blotting
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Specificity

Other Names

TBX21; TBET; TBLYM; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

NCBI Accession #

NP_037483.1

Other Accession #

NP_037483.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58328

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of A549 cell stained with TBX21 Antibody (N-term)(Cat# 102-12483).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX21 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).TBX21 immunoreactivity is localized to Nucleus significantly.

TBX21 Antibody (N-term) (Cat# 102-12483) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TBX21 antibody detected the TBX21 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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