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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 22800 |
| Protein Name / Synonyms | TC21 |
| Concentration (lot specific) | 0.69 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
TC21 antibody, RRAS2 antibody, teratocarcinoma oncogene antibody, ras-like protein TC21 antibody, ras-related protein R-Ras2 antibody, related RAS viral (r-ras) oncogene homolog 2 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
23 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 204 of TC21 (Uniprot ID# P62070)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using TC21(107-10927) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10927 diluted at 1:5000
A: mouse brain
12% SDS PAGE
107-10927 diluted at 1:5000
Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10927 diluted at 1:5000
A: A431
12% SDS PAGE
107-10927 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.