Anti-TCF25 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9BQ70
Gene Id	22980
Gene Symbols	TCF25
Protein Name / Synonyms	TCF25 (N-term)
Clonality	Polyclonal
Preparation	This TCF25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the N-terminal region of human TCF25.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM].

Specificity

Predicted Reactivity

Mouse

Other Names

TCF25; KIAA1049; NULP1; Transcription factor 25; Nuclear localized protein 1

NCBI Accession #

NP_055787.1

Other Accession #

Q8R3L2; NP_055787.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

76667

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of A549 cell stained with TCF25 Antibody (N-term)(Cat# 102-13352).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TCF25 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).TCF25 immunoreactivity is localized to Cytoplasm significantly.

TCF25 Antibody (N-term) (Cat# 102-13352) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TCF25 antibody detected the TCF25 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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