Synonyms
99D8.1 antibody, CCT-beta antibody, CCTB antibody, MGC142074 antibody, MGC142076 antibody, PRO1633 antibody, TCP-1-beta antibody, CCT2 antibody, "T-complex protein 1, beta subunit antibody", T-complex protein 1 subunit beta antibody, "chaperonin containing t-complex polypeptide 1, subunit 2 antibody", "chaperonin containing t-complex polypeptide 1, beta subunit antibody", "chaperonin containing TCP1, subunit 2 (beta) antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 277 and 503 of TCP1 beta (Uniprot ID# P78371)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TCP1 beta(107-11017) antibody at 1:100 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11017 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
B: Raji
7.5% SDS PAGE
107-11017 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.