Anti-TCP1 theta (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	10694
Protein Name / Synonyms	TCP1 theta, Internal
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C21orf112 antibody, Cctq antibody, D21S246 antibody, KIAA0002 antibody, PRED71 antibody, CCT8 antibody, T-complex protein 1 subunit theta antibody, TCP-1-theta antibody, renal carcinoma antigen NY-REN-15 antibody, "T-complex protein 1, theta subunit antibody", CCT-theta antibody, "chaperonin containing TCP1, subunit 8 (theta) antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

60 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 111 and 367 of TCP1 theta (Uniprot ID# P50990)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10555 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using TCP-1 theta (107-10555) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
7.5% SDS PAGE
107-10555 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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