Anti-TERT (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14746
Gene Id	7015
Gene Symbols	TERT
Protein Name / Synonyms	TERT (Center)
Clonality	Polyclonal
Preparation	This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-656 amino acids from the Central region of human TERT.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, FC
Research Area	Angiogenesis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.

Specificity

Other Names

TERT; EST2; TCS1; TRT; Telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2

NCBI Accession #

NP_001180305.1;NP_937983.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

126997

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT(arrow) was detected using the purified Pab.
	TERT Antibody (Center) (Cat# 102-12313) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-12313 TERT (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
	Fluorescent confocal image of Hela cell stained with TERT Antibody (Center)(Cat# 102-12313).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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