Anti-TGF alpha Antibody

Catalog #: DS-PB-01936

Rabbit Anti-Human TGF alpha

Product Description

Specifications

Size	0.1 mg
Estimated Lead Time	1-2 weeks
Species	Human
Specificity	This product is specific for human TGF-alpha (transforming growth factor alpha), a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS). TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.
Host Species	Rabbit
Protein Name / Synonyms	TGF alpha
Preparation	Antisera to human TGF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.
Concentration (lot specific)	IgG concentration 1.0 mg/ml
Recommended Applications	ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Reconstitution	Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Shipping Type	Ambient temperature
Storage	-20°C

Description

Immunohistology:

*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

Format Type:

Purified

Preparation

Antisera to human TGF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.

Immunogen:

E.cloli derived recombinant human TGF-alpha

Preservative:

None present

Expiration:

12 months from the date of shipment when stored properly.

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