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ISO 13485:2016 cGMP
Anti-TIMP-3 Antibody
Catalog #: 130-00030
Mouse Anti-Human TIMP-3 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | CAG46779 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from TIMP1. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= at least detecting 0.5ug/ml), Western Blotting (recommended work dilution= 1-2 µg/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5?g/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system. |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
TIMP1 is a glycoprotein expressed in several tissues of organisms. It is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
Specificity
The antibody shows specific binding to its immunogen. Specific binding to recombinant and native TIMP-1 has not been tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
References
- Hornebeck W (2004). Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival. Pathol. Biol. 51 (10): 56973.
- Gardner J, Ghorpade A (2004). Tissue inhibitor of metalloproteinase (TIMP-1): the TIMPed balance of matrix metalloproteinases in the central nervous system. J. Neurosci. Res. 74 (6): 8016.
Expiration:
12 months from the date of shipment when stored properly.