Mouse anti-TIMP-3 Biotinylated Antibody, 50 ug
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | CAG46779 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was recombinant protein derived from TIMP1. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Isotype | IgG1 |
| Recommended Applications | ELISA (recommended work dilution= at least detecting 0.5 µg/ml), Western Blotting (recommended work dilution= 1-2 µg/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5 µg/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system. |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
TIMP1 is a glycoprotein expressed in several tissues of organisms. It is a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody shows specific binding to its immunogen. Specific binding to recombinant and native TIMP-1 has not been tested.
References
- Hornebeck W (2004). Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival. Pathol. Biol. 51 (10): 56973.
- Gardner J, Ghorpade A (2004). Tissue inhibitor of metalloproteinase (TIMP-1): the TIMPed balance of matrix metalloproteinases in the central nervous system. J. Neurosci. Res. 74 (6): 8016.
Expiration:
12 months from the date of shipment when stored properly.
