Rabbit Anti-Human TIMP1 Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_003254 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human tissue inhibitor of metalloproteinases 1. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:3.2x10e5(at least detecting 32ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein TIMP1. The membrane blot was probed with antiTIMP1 primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular TIMP1 from human and mouse cells (23 kDa). |
| Antigen Catalog Number | 230-00003 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
TIMP1 (Tissue inhibitor of metalloproteinases 1) is a glycoprotein expressed from the several tissues of organisms. It is one member of TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function.
Specificity
The rabbit anti-human TIMP1 antibody detects specifically target derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
Related Products
Rat TIMP-1 ELISA Kit For Serum, Plasma and Cell Culture Supernatant (cat# ELRTIMP1-001) Recombinant Human Tissue Inhibitor of Metalloproteinase 1 (TIMP1), Western Blot Control (cat# 230-00003-WBC)Mouse TIMP-1 ELISA Kit For Cell and Tissue Lysate ( cat# ELM-TIMP1-001C)
References
- MN Holten-Andersen et al. (1999) Quantitation of TIMP-1 in plasma of healthy blooddonors and patients with advanced cancer . British Journal of Cancer 80(3/4), 495503
Expiration:
12 months from the date of shipment when stored properly.
