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Anti-TIMP3 Antibody, Biotinylated
Catalog #: 130-00010B-50
Chicken anti-TIMP3 Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Chicken |
| Accession Number | NP_000353 |
| Immunogen | Immunogen was synthetic peptide derived from Human TIMP3. This antibody was produced from a chicken immunized with the immunogen. The IgY fraction was purified from collected egg followed by in-house affinity chromatography. |
| Immunogen Sequence | CIRQKGGYCSWYRGWAPPDKS |
| Isotype | IgY |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000-2, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases-1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metallo-proteinase. TIMP-1 was found to be identical to EPA (erythroidpotentiation activity). Parathyroid hormone has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart and low levels of TIMP-4 are expressed in liver, brain, lung, thymus and spleen.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens as did in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat TIMP3 has not yet been tested.
Related Products
Rabbit Anti-human TIMP3, Cat# RB-15-0001Recombinant Human TIMP3, Western Blot Control, Cat# RB-15-0001
References
- Apte, S.S., et al. 1994. Gene encoding a novel murine tissue inhibitor of metalloproteinases (TIMP), TIMP-3, is expressed in developing mouse epithelia, cartilage, and muscle, and is located on mouse chromosome 10. Dev. Dyn. 200: 177-197.
- Silbiger, S.M., et al. 1994. Cloning of cDNAs encoding human TIMP-3, a novel member of the tissue inhibitor of metalloproteinase family. Gene 141:293-297
Expiration:
12 months from the date of shipment when stored properly.