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Anti-TNI Antibody
Catalog #: 130-10498
Mouse anti-TNI (1H5-F4-E11) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P19429 |
| Gene Id | 7137 |
| Gene Symbols | TNNI3 TNNC1 |
| Protein Name / Synonyms | Troponin I, cardiac muscle (Cardiac troponin I) |
| Clone | 1H5-F4-E11 |
| Immunogen | Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:320, 000)Western Blotting (recommended work dilution= 1:1, 000-5, 000), |
| Antigen Catalog Number | 230-00047 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution .Reconstitute the antibody with sterile water to a final concentration of 1mg/ml. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character.
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.