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Anti-TNi Antibody, Biotinylated
Catalog #: 130-10066B-50
Rabbit anti-TNi Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13489 |
| Gene Id | 330 |
| Gene Symbols | BIRC3 API2 MIHC RNF49 |
| Clonality | Polyclonal |
| Immunogen | The Immunogen was recombinant protein derived from human TnI. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:160, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein TnI. The membrane blot was probed with anti-TnI primary antibody (1 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular TNi from human and mouse cells (27 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Troponin I, one of 3 subunits that form the troponin complex of the thin filaments of striated muscle, is the inhibitory component of troponin. Troponin, in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) . Unlike other contractile proteins, the cardiac isoform of troponin I (TnIc) is expressed only in cardiac muscle and therefore offers a model for cardiac-specific expression. It is also subject to developmental regulation with increased expression occurring at the time of birth. TnIc, a sensitive and specific marker of myocardial cell injury, is useful in diagnosing and assessing prognosis in acute coronary syndromes. Studies report that TnIc is elevated in severe heart failure and may predict adverse outcomes.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to Human TnI. Cross reactivity with mouse and rat TnI was not tested.
Related Products
Mouse Anti-Troponin I-Cardiac (catalog #: MD-14-0977)
References
- Schulz O, et al.. (2010) High-sensitive cardiac troponin I (hs-cTnI) values in patients with stable cardiovascular disease: an initial foray. Clin Chim Acta. 411(11-12):812-7.
- Kimura A,et al.. (1997) Mutations in the cardiac troponin I gene associated with hypertrophic cardiomyopathy. Nat Genet. 16(4):379-82.
Expiration:
12 months from the date of shipment when stored properly.