Anti-TOM1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	O60784
Gene Id	10043
Gene Symbols	TOM1
Protein Name / Synonyms	TOM1, target of Myb protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Autophagy, Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene was identified as a target of the v-myb oncogene. The encoded protein shares its N-terminal domain in common with proteins associated with vesicular trafficking at the endosome. It is recruited to the endosomes by its interaction with endofin. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

48kDa/50kDa/53kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 314-493 of human TOM1 (NP_001129204.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TOM1 antibody (144-61471) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TOM1 Polyclonal Antibody (144-61471)

Immunofluorescence analysis of 293T cells using TOM1 antibody (144-61471) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - TOM1 Polyclonal Antibody (144-61471)

Expiration:

12 months from the date of shipment when stored properly.

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