Anti-TOP1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P11387
Gene Id	7150
Gene Symbols	TOP1
Protein Name / Synonyms	TOP1 (N-term)
Clonality	Monoclonal
Preparation	This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Specificity

Other Names

DNA topoisomerase 1, DNA topoisomerase I, TOP1

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

90726

Recommended Dilutions

WB: 1:1000

Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (Cat# 102-10479). 102-10479 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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