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Anti-TP53 Antibody
Catalog #: 144-61510
Phospho-TP53-S33 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04637 |
| Gene Id | 7157 |
| Gene Symbols | TP53 |
| Protein Name / Synonyms | TP53, BCC7, LFS1, P53, TRP53, tumor protein p53 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Apoptosis, Cancer, p53 Signaling, Autophagy, DNA Damage |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
23-43kDa
Observed MW
55kDa
Immunogen
A synthetic phosphorylated peptide around S33 of human TP53 (NP_000537.3).
Modification
Phospho S33
Storage
-20°C
Images
Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody (144-61510) at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using Phospho-TP53-S33 antibody (144-61510) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.