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Anti-TPI1 (N-term) Antibody

Catalog #: 102-15224

Rabbit Anti-TPI1 (N-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P60174
Gene Id
7167
Gene Symbols
TPI1
Protein Name / Synonyms
TPI1 (N-term)
ClonalityPolyclonal
PreparationThis TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-96 amino acids from the N-terminal region of human TPI1.
Concentration (lot specific)
2.0 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Research AreaCardiovascular Disease
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

TPI1 is an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis.

Specificity

Predicted Reactivity
Rat, Bovine, Pig, Monkey, Rabbit
Other Names
TPI1; TPI; Triosephosphate isomerase; Triose-phosphate isomerase
NCBI Accession #
NP_000356.1;NP_001152759.1;NP_001244955.1
Other Accession #
P48500; P00939; Q29371; P17751; Q60HC9; Q5E956

Format

Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30791
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
Western blot analysis of TPI1 Antibody (N-term) (Cat# 102-15224) in Y79(lane 1),CEM(lane 2) cell line and mouse brain tissue(lane 3) lysates (35ug/lane). TPI1 (arrow) was detected using the purified Pab.(2ug/ml)
Western blot analysis of TPI1 (arrow) using rabbit polyclonal TPI1 Antibody (N-term) (Cat# 102-15224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPI1 gene.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TPI1 Antibody (N-term) (Cat# 102-15224) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.