Anti-TPRKB Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	51002
Protein Name / Synonyms	TPRKB
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

TPRKB antibody, PRPK (p53-related protein kinase)-binding protein antibody, TP53RK-binding protein antibody, PRPK-binding protein antibody, TP53RK binding protein antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

20 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 175 of TPRKB (Uniprot ID# Q9Y3C4)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TPRKB(107-10644) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using TPRKB(107-10644) antibody at 1:250 dilution.

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
107-10644 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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