Anti-Transglutaminase (TGM2) (Center K444) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P21980
Gene Id	7052
Gene Symbols	TGM2
Protein Name / Synonyms	Transglutaminase (TGM2) (Center K444)
Clonality	Polyclonal
Preparation	This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.

Specificity

Other Names

TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; Transglutaminase H; Transglutaminase-2

NCBI Accession #

NP_004604.2;NP_945189.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

77329

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	TGM2 Antibody (Center K444) (Cat# 102-17645) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).
	Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444)(Cat# 102-17645).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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